Membrane:Article Title: Human Platelet Lysate-Derived Nanofibrils as Building Blocks to Produce Free-Standing Membranes for Cell Self-Aggregation.
Article Snippet: .. To assess the membrane stability for cell culture purposes, membranes formed with 7.5 μL of PLMA fibril suspension were rehydrated in Minimum Essential Medium α (αMEM, Thermo Fisher Scientific) supplemented with sodium bicarbonate (2.2 g·mL−1, Sigma-Aldrich), 10% (v/v) heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific), and 1% (v/v) antibiotic/antimycotic (10,000 units·mL−1 penicillin, 10,000 μg·mL−1 streptomycin, and 25 μg·mL−1 amphotericin B, Thermo Fisher Scientific). ..
Cell Culture:Article Title: Human Platelet Lysate-Derived Nanofibrils as Building Blocks to Produce Free-Standing Membranes for Cell Self-Aggregation.
Article Snippet: .. To assess the membrane stability for cell culture purposes, membranes formed with 7.5 μL of PLMA fibril suspension were rehydrated in Minimum Essential Medium α (αMEM, Thermo Fisher Scientific) supplemented with sodium bicarbonate (2.2 g·mL−1, Sigma-Aldrich), 10% (v/v) heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific), and 1% (v/v) antibiotic/antimycotic (10,000 units·mL−1 penicillin, 10,000 μg·mL−1 streptomycin, and 25 μg·mL−1 amphotericin B, Thermo Fisher Scientific). ..
Article Title: Upturn Strategies for Arachidonic Acid-Induced MC3T3-E1—625 nm Irradiation in Combination with NSAIDs: Dissipating Inflammation and Promoting Healing
Article Snippet: .. MC3T3-E1 were cultured in the Minimum Essential Medium α (αMEM, Thermo Scientific, CA, USA) supplemented with 1% penicillin/streptomycin (Welgene, Gyeongsan, Republic of Korea) and 10% fetal bovine serum (FBS) (Atlas Biologicals, Fort Collins, CO, USA) in a 5% CO2 humidified atmosphere at 37 ◦C. ..
Article Title: Branched-Chain Amino Acid Catabolism Promotes Ovarian Cancer Cell Proliferation via Phosphorylation of mTOR
Article Snippet: Mycoplasma testing was done using Sigma LookOut Mycoplasma PCR Detection Kit and Lonza MycoAlert Mycoplasma Detection Kit. .. MOSE cells were cultured in minimum essential medium α (αMEM; MT10022CV, Thermo Fisher Scientific) supplemented with 10% FBS (21G267, Sigma-Aldrich), 2 mmol/L L-glutamine (TCG0063, VWR), 10 mg/mL insulin-transferrin-sodium selenite supplement (11074547001, Sigma-Aldrich), 1.8 ng/mL EGF (100-15, PeproTech), 100 U/mL penicillin–streptomycin (15140-122, Gibco), and 1 mg/mL gentamicin (30-005-CR, CellGro). .. The MOE cell lines (SCR shRNA and PTEN shRNA ) were maintained in similar media but with the addition of 18.2 ng/mL estradiol-17β (E1024, Sigma-Aldrich) and selection antibiotics.
Article Title: Compressed Prostate Cancer Cells Decrease Osteoclast Activity While Enhancing Osteoblast Activity In Vitro.
Article Snippet: Informed consent was obtained from all patients and The Ethical Review Board of the VU University Medical Center, Amsterdam, The Netherlands, approved the protocol (Protocol number: 2016/105) on 17 March 2016. .. Isolated hASCs were cultured in Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% Fetal Clone Serum 1 (FC1; HyClone, Logan, UT, USA) and 1% PSF (Sigma-Aldrich). ..
Suspension:Article Title: Human Platelet Lysate-Derived Nanofibrils as Building Blocks to Produce Free-Standing Membranes for Cell Self-Aggregation.
Article Snippet: .. To assess the membrane stability for cell culture purposes, membranes formed with 7.5 μL of PLMA fibril suspension were rehydrated in Minimum Essential Medium α (αMEM, Thermo Fisher Scientific) supplemented with sodium bicarbonate (2.2 g·mL−1, Sigma-Aldrich), 10% (v/v) heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific), and 1% (v/v) antibiotic/antimycotic (10,000 units·mL−1 penicillin, 10,000 μg·mL−1 streptomycin, and 25 μg·mL−1 amphotericin B, Thermo Fisher Scientific). ..
Modification:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Transfection:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Plasmid Preparation:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Staining:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Protease Inhibitor:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
cDNA Synthesis:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Real-time Polymerase Chain Reaction:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
SYBR Green Assay:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Polymerase Chain Reaction:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Next-Generation Sequencing:Article Title: Ndufa6 regulates adipogenic differentiation via Scd1
Article Snippet: .. Minimum essential medium α (αMEM, ThermoFisher Scientific, 12561056), Dulbecco’s modified Eagle medium (DMEM, ThermoFisher Scientific, 11965092), Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12, ThermoFisher Scientific, 11330032), foetal bovine serum (FBS, VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX, Aladdin Biochemical Technology, I106812), epidermal growth factor (EGF, GenScript, Z02972), fibroblast growth factor-basic (bFGF, GenScript Biotech, Z03016), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulphate (Sangon Biotech, A610494), Glutamax (ThermoFisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), jetPRIME® transfection reagent (Polyplus transfection, 114–15), MolPure endo-free plasmid mini kit (Shanghai YEASEN Biotech, 19021ES), oil red O (Sangon Biotech, A600395), Alizarin Red S staining kit (Solarbio Life Science, G1452), A939572 ( Scd1 inhibitor, Sigma, SML2356), RIPA buffer (Beyotime, Biotechnology, P0013B), protease inhibitor cocktail (MCE, HY-K0010), Skim (BD, 232100), anti-NDUFA6 (Biodragon-immunotech, BD-PN0922), anti-SCD1 (ABclonal, A16429), anti-β-ACTIN (ABclonal, AC026), HRP goat anti-rabbit IgG (ABclonal, AS014), PVDF membranes (Millipore, ISEQ00010), ECL (Millipore, WBKLS0500), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), ultrapure RNA kit (CWBIO, CW0581M), HiScript® III 1st strand cDNA synthesis kit (Vazyme Biotech, R312-02), Hieff UNICON® qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), second strand cDNA synthesis kit (Beyotime Biotech, D7172), and HIFI PCR mix for NGS (CWBIO, CW2648) were all commercially available. ..
Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
Isolation:Article Title: Compressed Prostate Cancer Cells Decrease Osteoclast Activity While Enhancing Osteoblast Activity In Vitro.
Article Snippet: Informed consent was obtained from all patients and The Ethical Review Board of the VU University Medical Center, Amsterdam, The Netherlands, approved the protocol (Protocol number: 2016/105) on 17 March 2016. .. Isolated hASCs were cultured in Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% Fetal Clone Serum 1 (FC1; HyClone, Logan, UT, USA) and 1% PSF (Sigma-Aldrich). ..
Radio Immunoprecipitation:Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
ATP Assay:Article Title: Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells
Article Snippet: .. Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific, 11330032), Minimum Essential Medium α (αMEM; Thermo Fisher Scientific, 12561056), jetPRIME transfection reagent (Polyplus-transfection, 114-15), fetal bovine serum (FBS; VivaCell Biotechnology, C04001), rosiglitazone (MedChemExpress, HY-17386), insulin (Shanghai Yuanye Bio-Technology, S12033), 3-isobutyl-1-methylxanthine (IBMX; Aladdin Biochemical Technology, I106812), type I collagenase (Sangon Biotech, A004194), penicillin G sodium salt (Sangon Biotech, A600135), streptomycin sulfate (Sangon Biotech, A610494), epidermal growth factor (EGF; GenScript, Z02972), basic fibroblast growth factor (bFGF; GenScript Biotech, Z03016), GlutaMAX (Thermo Fisher Scientific, 35050061), trypsin (Sangon Biotech, A100458), Oil Red O (Sangon Biotech, A600395), Ultrapure RNA Kit (CWBIO, CW0581M), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, A110-1-1), PD98059 (MedChemExpress, HY-12028), radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology, P0013B), polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010), Enhanced chemiluminescent reagent (ECL) (Millipore, WBKLS0500), ATP assay kit (Beyotime Biotechnology, S0026), Hieff UNICON qPCR SYBR green master mix (Shanghai YEASEN Biotech, 11198ES), total protein assay kit (Nanjing Jiancheng Bioengineering Institute, A045-4-2), HiFi PCR mix for next-generation sequencing (NGS) (CWBIO, CW2648), HiScript III first-strand cDNA synthesis kit (Vazyme Biotech, R312-02) and second-strand cDNA synthesis kit (Beyotime Biotechnology, D7172), Extracellular signal-regulated kinase (ERK) (Cell Signaling Technology, 4695), p-ERK (Cell Signaling Technology, 4370), C-Jun N-Terminal Kinase (JNK) (Cell Signaling Technology, 9252), p-JNK (Cell Signaling Technology, 4668), P38 (Cell Signaling Technology, 8690), p-P38 (Cell Signaling Technology, 4511), anti-slc25a5 (Biodragon Immunotech, BD-PN0419), anti-β-actin (ABclonal, AC026), anti-MTCO1 (ABclonal, A17889), anti-SDHB (Proteintech, 10620-1-AP), anti-ATP5A1 (Proteintech, 14676-1-AP), anti-UQCRC2 (Proteintech, 14742-1-AP), and anti-NDUFB8 (Proteintech, 14794-1-AP). ..
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